Stable Cell Line Generation Service

Choosing to outsource this application may not only save time and effort but can also provide benefits from technical expertise and facilities dedicated to the generation of stable cell lines.

Stable cell line generation service.

Stable expression can be influenced by the transfection method used. Unlike the short term protein expression observed using transient transfection approaches generating cell lines using lentiviral vectors enables long term protein expression studies. Major challenges for generation of stable cell lines are low transfection efficiency and or integration frequency. It is known that liposome reagents can be used to transfer dna into adherent cell lines.

Cell line development service options are available from several specialized providers. Creative biogene offers high quality fast turnaround and cost effective stable cell line generation service for protein expression gene knockdown or genome editing that meet your specific research needs. The transfection method determines the cell type for stable integration. Stable cell line generation cellomics technology llc provides high quality fast turnaround and cost effective stable cell line generation service for research biotechnology and pharmaceutical companies.

All stable cell lines are produced according to individual client specifications with efficient gene delivery technology able to produce stable expression in clonal cell lines in 28 days service description. Stable cell line generation stable cell line generation stable cell lines expressing a gene of interest or harboring knockout knockin or precise sequence changes e g. This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Stable cell lines with specific gene over expression or knock down are very helpful in gene function analysis target discovery target vali.

Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected plasmid dna or reporter gene constructs into the genome.