Stable Cell Line Development

This is a very common problem for many stable cell lines.

Stable cell line development.

The process of cell line development begins with the introduction of foreign dna encoding. This protocol describes the procedure for creating a stable cell line based on the expicho expression system for use in commercial bioproduction. Typical cell line workflow step 1. After several passages some stable cell lines may become unstable with their expression diminishing slowly.

Expicho s cells are transfected cloned and selected in expicho expression medium followed by the scale up in the bioproduction amenable expicho stable production medium. Stable cell line generation. With a one time fee and strong yield guarantees our stable cell line development service based on our proprietary cell line aims at offering high productivity at competitive price and fto without royalties. Gpex cell line development technology is a proven technology that generates high performance highly stable production cell lines.

That s why gibco products are designed to provide you with integrated stable cell line development solutions to simplify your development workflow and accelerate your processes to commercial manufacture. By applying single cell clonings from the selected stable pools selexis identifies high expressing clonal cell lines that are then evaluated for their. One is achieved by eukaryotic vectors that harbor elements for episomal maintenance in the nucleus of a transfected cell. Stable cell line development cell engineering group offers services for cell line development from dna sequences to rcb stability assessment.

We understand that cell line development involves multiple complex processes requiring consistency and reproducible results. Stable expression of target gene in cell lines overcomes the low transfection efficiency of transient expression and produces more target proteins. To date over 600 different mab and mab fusions and over 80 different recombinant proteins have been produced using the gpex system with 120 ongoing clinical trials utilizing therapeutic candidates developed. The development of high yield production cell lines starts with the cloning of the gene of interest into sure tech vectors that bear selexis sge selexis genetic elements.

Proteogenix strives to overcome the economic issues related to stable cell line generation by offering the most productive ip free cell lines on the market. You want to develop a stable cell line because it is stable in expressing the target protein. However not all stable cell lines are equally stable. Traceability of cell history and cell line generation process high productivity and robust stable cell line development platforms.

The jump in technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using phic31 integrase. These optimally designed dna expression vectors are then transfected into the fully documented selexis sure cho m cell line using the sure fection procedure.